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Home / Fish Probe

eFISH EGR1/5P15 Dual Color Probe

SKU : BHR15100171

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BioGenex Laboratories

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Mfr.No.	FP068-20X
Gene Name	EGR1/5P15
Fluorophore	Orange/Green
Chromosome	5
Intended Use	The BioGenex eFISH EGR1/5p15 Dual Color Probe is currently available for Researchuse only. eFISH EGR1/5p15 Dual Color Probe is designed to be used for the detection ofthe human EGR1 gene as well as chromosome 5 specific sequences in formalinfixed,paraffin-embedded tissue or cells by fluorescence in situ hybridization (FISH).BioGenex eFISH EGR1/5p15 Dual Color Probe comes in hybridization buffer. The probecontains green-labeled polynucleotides (Green: excitation at 503 nm and emission at 528nm, similar to FITC), which target the EGR1 gene, and orange-labeled polynucleotides(Orange:excitation at 547 nm and emission at 572 nm, similar to rhodamine), which targetsequences of chromosome 5 in the chromosomal region 5p15.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or withinindividual cells. FISH is also used for use in genetic counseling, medicine, and species identification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH EGR1/5p15 Dual Color Probe must be stored at 2-8°C protectedfrom light and is stable through the expiry date printed on the label.

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100171	FP068-20X	20 Test	$1793		Add to Cart

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