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Home / Fish Probe

eFISH CEN X/YQ12 Dual Color Probe

SKU : BHR15100235

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BioGenex Laboratories

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Mfr.No.	FP102-20X
Gene Name	CEN X/YQ12
Fluorophore	Orange/Green
Intended Use	The BioGenex eFISH CEN X/Yq12 Dual Color Probe is currently available for Research use only. eFISH eFISH CEN X/Yq12 Dual Color Probeis designed to be used for the detectionhuman chromosome X alpha-satellites as well aschromosome Yq12 specificsequences informalin-fixed, paraffin-embedded tissue or cellsby fluorescence in situ hybridization (FISH). eFISH BioGenexCEN X/Yq12 Dual Color Probeinhybridization buffer. The probe contains orangelabeledpolynucleotides (Orange: excitation at547 nm and emission at 572 nm, similar torhodamine), which target alpha-satellitesequences(DXZ1) in thechromosomal regionXp11.1-q11.1, and green-labeled polynucleotides(Green:excitation at 503 nm and emissionat 528 nm, similar to FITC), which targetsequences of the satellite III region (DYZ1) ofchromosome Y in the chromosomal region Yq12.
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or within individual cells. FISH is also used for use in genetic counseling, medicine, and speciesidentification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH CEN X/Yq12 Dual Color Probemust be stored at 2-8°C protectedfrom light and is stable through the expiry date printed on the label.

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SKU	Supplier No.	Size	Your price	Quantity
BHR15100235	FP102-20X	20 Test	$1793		Add to Cart

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