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Yeasen
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Yeasen
view supplier detailsCredit card payments now incur a 3% fee.
| Mfr.No. | 12214ES96, 12214ES24 | |||||||||||||||||||||||||||||||||||
| Description |
The Hieff NGS dsDNA Methyl Library Prep Kit for Illumina is a double-stranded DNA methylation library preparation kit developed for the Illumina high-throughput sequencing platform. This kit utilizes appropriate methylated adapters for ligation, followed by treatment with bisulfite or enzyme conversion, and then a PCR amplification step to construct methylated libraries for sequencing on the Illumina platform. All components of the kit undergo quality control and functional validation to ensure stable library output, supporting various types of DNA samples including gDNA, cfDNA, and FFPE. The recommended input DNA amount for this kit is 10 ng to 1000 ng, and it is compatible with commonly used conversion protocols. Workflow   The DNA library prepared with the Hieff NGS dsDNA Methyl Library Prep Kit using 10ng of cfDNA. In the graph, the red line represents the DNA library constructed through enzymatic conversion with 10 cycles of PCR amplification, while the blue line represents the DNA library constructed through bisulfite conversion with 12 cycles of PCR amplification. |
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| Precautions |
1. For your safety and health, wear a lab coat and disposable gloves while handling. 2. Before use, thaw all components of the kit at room temperature. After thawing, mix thoroughly by inverting several times, briefly centrifuge, and keep on ice until ready to use. 3. When preparing reaction mixtures for each step, it is recommended to mix by pipetting up and down or gently vortexing. Vigorous shaking may result in decreased library yield. 4. To avoid cross-contamination of samples, it is recommended to use filter tips and to change tips between different samples. 5. Perform all steps in a PCR machine with a heated lid, and preheat the PCR machine to the reaction temperature before use. 6. PCR products are highly susceptible to aerosol contamination, which can affect the accuracy of experimental results. It is recommended to physically separate the PCR setup area from the PCR product purification and detection area. Use dedicated pipettes and equipment for each area, and regularly clean each area with 0.5% sodium hypochlorite or 10% bleach to maintain cleanliness. 7. This product is for research use only! |
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| Components |
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| Combination SKU | Supplier No. | Size | Your price | Quantity | |
|---|---|---|---|---|---|
| BHS20800026-1 | 12214ES96 | 96 T | $4205 |
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| BHS20800026-2 | 12214ES24 | 24 T | $1325 |
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