Hieff NGS DNA Spike in Mix for CUT&Tag

Mfr.No.	12596ES48
Description	Hieff NGS DNA Spike-in Mix for CUT&Tag (5 ng/μL) is derived from three sequences of Lambda DNA from E. coli, with lengths of 230 bp, 250 bp, and 300 bp, and a molar concentration ratio of approximately 1:3:10. This standard is primarily used for normalization and quantitative analysis of CUT&Tag sequencing data under different treatment conditions or varying cell states. Add 1 μL per 100,000 cells, with adjustments possible based on the binding capacity and abundance of the target protein.
Important Notes	1. The DNA Spike-in Mix provided by this product contains three sequences derived from E. coli λDNA, with a concentration of 5 ng/μL and lengths of 230 bp, 250 bp, and 300 bp, in a molar concentration ratio of approximately 1:3:10. For experiments, it is recommended to dilute the DNA Spike-in Mix with TE buffer to a concentration of 5 pg/μL. 2. This product is primarily used for normalization and quantitative analysis of sequencing data under different treatment conditions or cell states and is an optional component. 3. This product is for research use only!