Hieff NGS DNA Spike-in Mix for CUT&Tag (5 ng/?L) is derived from three sequences of Lambda DNA from E. coli, with lengths of 230 bp, 250 bp, and 300 bp, and a molar concentration ratio of approximately 1:3:10. This standard is primarily used for normalization and quantitative analysis of CUT&Tag sequencing data under different treatment conditions or varying cell states. Add 1 ?L per 100,000 cells, with adjustments possible based on the binding capacity and abundance of the target protein.
Product Components
Component No.
Component name
12596ES48
12596-A
DNA Spike in Mix?5 ng/?L?
10 ?L
Shipping and Storage
Stored at -25~-15?for one year.
Figures
Notes
1. The DNA Spike-in Mix provided by this product contains three sequences derived from E. coli ?DNA, with a concentration of 5 ng/?L and lengths of 230 bp, 250 bp, and 300 bp, in a molar concentration ratio of approximately 1:3:10. For experiments, it is recommended to dilute the DNA Spike-in Mix with TE buffer to a concentration of 5 pg/?L.
2. This product is primarily used for normalization and quantitative analysis of sequencing data under different treatment conditions or cell states and is an optional component.