Uracil-Specific Excision Reagent (1 U/uL)

The Uracil-Specific Excision Reagent (referred to as USER) is an enzyme cocktail consisting of Uracil DNA Glycosylase (UDG) and Endonuclease VIII (Endo VIII). It is capable of generating single-nucleotide gaps specifically at uracil locations. UDG facilitates the cleavage of the uracil base, resulting in the formation of an AP site (apurinic/apyrimidinic site) while maintaining the integrity of the phosphodiester sugar-phosphate backbone. The endonuclease activity of Endo VIII cleaves the phosphodiester bonds at both the 3? and 5? ends of the apurinic site, thereby releasing the deoxyribose sugar that is lacking a base.

Features

No residual nucleases or RNases

High excision efficiency

Applications

RNA strand-specific library construction

Uracil-specific excision-mediated cloning (USER-LIC)

DNA assembly, such as the assembly of TALE monomers

Serial construction of multiple cDNA libraries from single cells

Specifications

Components

Shipping and Storage

The products should be stored at -25? ~ -15? for 1 year.

Figures

Figure1. Comparison of excision effects

Note: 10 pmol of double-stranded DNA containing dUTP was excised using Yeasen's USER enzyme and a comparable product from Supplier A. The results from agarose gel electrophoresis showed that the excision effects of Yeasen's USER enzyme were consistent with those of Supplier A

Figure2. Comparison of colony counts for USER-LIC cloning

Note: A 600 bp DNA fragment was ligated using Yeasen's USER enzyme and a comparable product from Supplier A to construct a vector, which was then transformed into E. coli and cultured. The number of colonies on the culture plate was observed, and the results indicated that the USER-LIC cloning efficiency using Yeasen's USER enzyme was superior to that of Supplier A.

Documents:

Safety Data Sheet

14537_MSDS_Ver.EN20241210.pdf

Manuals

14537_Manual_Ver.EN20241210.pdf