Uracil DNA Glycosylase (UDG), Heat-Labile (Glycerol free) (1 U/uL)

Mfr.No.

10707ES76

Description

UDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP. Compared to regular UDG, Heat-Labile UDG avoids the potential residual activity that regular UDG may have after inactivation, which could degrade dU-containing amplification products at room temperature.

Specifications

Unit Definition	The amount of enzyme required to completely degrade 1 μg of dsDNA containing uracil within 30 minutes at 25°C is defined as 1 activity unit (U).
Deactivation method	94℃, 2-5 minutes.

Components

No.	Name	10707ES60	10707ES76	10707ES90	10707ES92
		(100 U)	(500 U)	(5 KU)	(10 KU)
10707	Uracil DNA Glycosylase (UDG, Glyceol-free), (1 U/μL)	100 μL	500 μL	5 mL	10 mL

Caution

1. For your safety and health, please wear a lab coat and disposable gloves.
2. Thermosensitive UDG is active in most PCR reaction buffers.

Heat Inactivation

94℃, 2-5 minutes.

Potential Application

1. Remove aerosol contamination from PCR products containing dU.
2. Remove single-stranded or double-stranded DNA uracil bases.

Storage	Store at 4°C, valid for 6 months.
Shipping	Ice pack transport.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHZ20800051-1	10707ES76	500 U	$395		Add to Cart

Uracil DNA Glycosylase (UDG), Heat-Labile (Glycerol free) (1 U/uL)

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