UCF.ME Uracil DNA Glycosylase (UDG/UNG), heat-labile, 1 U/uL

Description
Review(s)

Mfr.No.

14466, 14466ES60, 14466ES76, 14466ES96

Description

UCF.ME Uracil DNA Glycosylase derived from psychrophilic marine bacteria, it is an ultra-low residual heat-labile UDG enzyme known as UCF.ME^TM. This product can prevent the residual activity of conventional UDG enzymes after inactivation from degrading dU-containing amplification products at room temperature. It also prevents background bacteria present in molecular enzymes from causing false positives in PCR results. Therefore, the introduction of UCF.ME^TM ultra-low residual thermolabile UDG enzyme is crucial for accurately identifying infectious pathogens and assisting in the clinical diagnosis of infections and infectious diseases.

Features

Ultra-low residual of UCF.ME^TME. coli genomic DNA residue <0.1 copies/ U;

Low nuclease residueNo residual exonuclease, nicking enzyme, or RNase;

Strong digestion capability0.05 U/T can digest 10⁵ copies/T dU-DNA products;

Good thermolabilityComplete inactivation under any condition of 50? for 10 minutes, 55? for 5 minutes, or 95? for 5 minutes;

Compatible with RT-qPCR reaction systemsNo impact on the detection system even at high input levels (2U/20?L reaction system).

Specifications

Source	Recombinant E. coli with UDG gene from psychrophilic marine bacteria
Storage Buffer	20 mM Tris-HCl, 100 mM KCl, 0.1mM EDTA,1mM DTT, 0.5% Tween-20, 0.5% NP-40, 50% Glycerol, pH8.0 ± 0.2 @ 25?
Unit Definition	One unit (U) is defined as the amount of enzyme that required to catalyze the hydrolysis of 1 ?g dU-containing dsDNA in 30 minutes at 25°C
Heat Inactivation	55? for 5 min; 50? for 10 min

Components

Name

14466ES60

100 U

14466ES76

500 U

14466ES96

10,000 U

UCF.ME^TM Uracil DNA Glycosylase (UDG/UNG), heat-labile, 1 U/?L

100 ?L

500 ?L

10 mL

Storage

The product should be stored at -25~-15°C for 2 years.

Figures

Figure 1. 14466ES protein purity (SDS-PAGE) detection results. Protein Purity?95%

Figure 2. 14466ES Nuclease residue test results. No Residual Nucleases, No Residual Exonucleases, Nicking Enzymes, or RNases.

Figure 3. 14466ES E.coli genomic DNA residue test results. E.coli genomic DNA residue< 0.1 copies/ U, the residue levels were significantly lower than the conventional UDG.

Figure 4. Strong digestive capability. 0.05 U/T is sufficient to digest 10^5 copies/T of dU-DNA products. 14466ES was used to prepare qPCR mix, while the control group of qPCR mix without UDG enzyme could completely digest 10⁵ copies/T dU-DNA products.