UCF.ME^TM Salt Active UltraNuclease GMP grade (250 U/uL)

Mfr.No.

20159ES25, 20159ES60, 20159ES90, 20159ES80

Description

Salt Active UltraNuclease is a nonspecific endonuclease and has optimum activity at high salt concentration. Salt can play an important role in minimizing aggregation of protein or virus, which would be helpful for dissociation of DNA & RNA from proteins or other cellular components. In high salt conditions, enzymes are more likely to access the released DNA & RNA and degrade them. Nuclease which has high activity at high salt would be more effective to improve purification processes. The Salt Active UltraNuclease can be used to reduce the viscosity of cell supeatant and cell lysate, increase purification efficiency in high salt condition.

The enzyme can also reduce host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing.

This product is produced by GMP process requirements and provided in a liquid form with the following features.
- High purity (≥ 98%)
- Animal-free production
- Low Endotoxin
- High activity at high salt conditions
- Supported by ELISA Kit

Notes

Recommended operating conditions

Condition	Optimal	Effective
Mg2+/Mn2+	10~50mM	1~100mM
pH	8	6.5 ~10
Temperature	37℃	4~42℃
Na+/K+	500mM	300~1200mM

Recommended Usage

Condition	Suggested usage
Protein	100U/mL
Cell lysate	500U/mL
Cell extract	1000U/mL
Viscosity reduction	50U/mL

AAV Production Case

Supplier	Condition (100U/mL, 37℃, 1h)
M*	400mM NaCl
M*	400mM NaCl,1% Tween
Yeasen 20159ES	400mM NaCl
Yeasen 20159ES	400mM NaCl,1% Tween

Background Info

Expression Host	Recombinant, expressed in Escherichia coli
Molecular Weight	24.7kDa
Isoelectric point	9.61
Purity	≥ 99%
Storage Buffer	25 mM Tris-HCl，5 mM MgCl2，500 mM NaCl，50% glycerol
Unit Definition	The definition of one activity unit (U) is the amount of enzyme that causes a △A260=1.0 in 30 minutes at 37℃ in the excess of substrate at the certain condition.

Storage	The product should be stored at -25 ~ -15℃ for two years. If the product is opened and has been stored at 4℃ for more than one week, we recommend filtering the product to prevent microbial contamination.
Format	Endonuclease

Protocals And Manuals	Manual

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Combination SKU	Supplier No.	Size	Your price
BHZ20800049-1	20159ES25	25 KU	$195	Add to Cart
BHZ20800049-2	20159ES60	100 KU	$585	Add to Cart
BHZ20800049-3	20159ES90	5000 KU (20 mL)	$8805	Add to Cart
BHZ20800049-4	20159ES80	1000 KU (4 mL)	$2605	Add to Cart

UCF.ME^TM Salt Active UltraNuclease GMP grade (250 U/uL)

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