T7 RNA Polymerase GMP grade (50 U/uL)

Mfr.No.

10624ES90, 10624ES97

Description

This product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA.

Note: G* is the first base of the RNA transcript.

This product is produced in accordance with GMP regulations and provided in liquid form.

Feature
Synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template
Higher yield and easy to operate
Lower endotoxins
Tested for the absence of endonucleases, exonucleases, RNases

Application
Radiolabeled RNA probe preparation
RNA generation for studies of RNA structure, processing and catalysis
Expression control via anti-sense RNA
mRNA, sgRNA synthesis

Specification

Source	Expressed in E. coli with a recombinant T7 RNA Polymerase gene
Optimum Temperature	37℃
Storage Buffer	50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v/v) glycerin，pH7.9 at 25℃
Unit Definition	The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.

Components

Components No.	Name	10624ES90 (5,000 U)	10624ES97 (50,000 U)
10624	T7 RNA Polymerase GMP-grade (50 U/μL)	100 μL	1 mL

Storage	Stored at -15℃ ~ -25℃ for one year.
Shipping	T7 RNA Polymerase GMP-grade products are shipped with dry ice.
Format	Polymerase

Protocals And Manuals	Safety Data Sheet Manual

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHZ20800045-1	10624ES90	5000 U	$70		Add to Cart
BHZ20800045-2	10624ES97	50000 U	$565		Add to Cart

T7 RNA Polymerase GMP grade (50 U/uL)

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