JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
orders@biohippo.com

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Enzymes, Inhibitors & Substrates

T7 Endonuclease I

SKU : BHZ10900004

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Request More Infos

Applied Biological Materials (abm) Inc.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	E028
Description	T7 Endonuclease I recognizes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA, Holliday structures and/or junctions, as well as nicked double-stranded DNA. This enzyme has a preference for single stranded over double stranded DNA with cleavage occurring at the first, second or third phosphodiester bond that is 5´ to the mismatch.
Description	Mutation detection assayDetect and cleave heteroduplex and/or nicked DNACleave DNA randomly for use in shotgun cloning
Notes	One unit is defined as the amount of T7 Endonuclease I that linearizes over 90% of 1 µg of supercoiled cruciform DNA in a 50 µl volume in 1 hour at 37°C.
Concentration	10 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	1000 U (100 μl)

Storage	Store all components at -20°C. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. All components are stable for 1 year from the date of shipping when stored and handled properly.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 25 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.15% Triton® X-100 and 50% (v/v) Glycerol.
Format	Endonuclease
Concentration	10 U/μl

Supporting Protocol	T7 Endonuclease I Data Sheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHZ10900004	E028	100µl	$125		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.