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Home / Enzymes, Inhibitors & Substrates

T4 RNA Ligase 2 (Truncated)

SKU : BHZ10900063

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E012
Description	T4 RNA Ligase 2 (Truncated) catalyzes the formation of a phosphodiester bond between a pre-adenylated 5’ phosphate (DNA or RNA) and the 3’ hydroxyl of RNA. This truncated enzyme contains the first 249 amino acids and does not require ATP for ligation but does require a pre-adenylated 5’ terminal donor. In cases where the substrate is pre-adenylated on the 5’ end, T4 RNA ligase 2 (Truncated) is a better choice compared to the full-length enzyme because it generates less side-reaction ligation products as it cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA.
Description	Ligating pre-adenylated DNA or RNA to the 3’ end of RNA ; Ligating single stranded adenylated primers to RNA for cDNA library and generation and for strand-specific cDNA library construction
Notes	One unit is defined as the amount of T4 RNA Ligase 2 (Truncated) required to ligate 50% of 0.4 μg of an equimolar mix of a single-stranded 5’ FAM-labelled 17-mer RNA to the 5’ pre-adenylated end of a 18-mer DNA when both 17-mers are annealed to a complementary 35-mer DNA strand in a total reaction volume of 20 µl in 30 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	200 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	100 μl

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT and 50% (v/v) Glycerol.
Format	Ligase
Concentration	200 U/μl

Protocol	E012 Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900063	E012	100µl	$125		Add to Cart

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