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Home / Enzymes, Inhibitors & Substrates

T4 RNA Ligase 2 (dsRNA Ligase)

SKU : BHZ10900055

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E011
Description	T4 RNA Ligase 2 (dsRNA Ligase) catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining. Unlike T4 RNA Ligase 1 which ligates single-stranded RNA, T4 RNA Ligase 2 joins knicks on double-stranded RNA and can also ligate the 3’OH of RNA to the 5’ phosphate of DNA in a double stranded structure.
Description	Ligating nicks in double-stranded RNA ; Ligating the 3’OH of RNA to the 5’phosphate of DNA
Notes	One unit is defined as the amount of T4 RNA Ligase 2 that ligates 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µl in 30 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	10 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	100 μl

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 35 mM (NH4)2SO4 and 50% (v/v) Glycerol.
Format	Ligase
Concentration	10 U/μl

Protocol	E011 Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900055	E011	100µl	$81.80		Add to Cart

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