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Home / Enzymes, Inhibitors & Substrates

T4 RNA Ligase 1 (ssRNA Ligase)

SKU : BHZ10900028

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E010
Description	T4 RNA Ligase 1 catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond on a broad range of substrates including single-stranded RNA and DNA, oligoribonucleotides, oligodeoxynucleotides, as well as numerous nucleotide derivatives. The phosphodiester bond is created between a 5′-phosphoryl-terminated nucleic acid donor and a 3′-hydroxyl-terminated nucleic acid acceptor in a template-independent manner.
Description	RNA 3'-termini labeling with cytidine 3',5'-bis [alpha-32P] phosphate ; Intermolecular and intramolecular joining of RNA and DNA ; Incorporation of unnatural amino acids into proteins ; Synthesis of oligoribonucleotides and oligodeoxyribonucleotides ; 5′- and 3′-end mapping of mRNA
Notes	One unit is defined as the amount of T4 RNA Ligase 1 that converts 1 nmole of 5´-[32P]rA16 into a phosphatase-resistant form in 30 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	10 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	100 μl

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT and 50% (v/v) Glycerol.
Format	Ligase
Concentration	10 U/μl

Supporting Protocol	E010 Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900028	E010	100µl	$63.25		Add to Cart

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