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Home / Enzymes, Inhibitors & Substrates

T4 Polynucleotide Kinase

SKU : BHZ10900074

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E006
Description	T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-hydroxyl terminus of double and single-stranded RNA and DNA, oligonucleotides or nucleoside 3´-monophosphates. The enzyme is also capable of catalyzing the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
Description	Labelling 5’-termini of DNA or RNA to be used as: ; o primers for DNA sequencing ; o primers for PCR ; o probes for hybridization ; o probes for transcript mapping ; o markers for gel electrophoresis ; Addition of 5'-phosphates to oligonucleotides, PCR products, and DNA or RNA prior to ligation ; Removal of 3´-phosphoryl groups
Notes	One unit is defined as the amount of T4 Polynucleotide Kinase that catalyzes the incorporation of 1 nmol γ-phosphate from ATP to the 5’-hydroxyl termini of micrococcal nuclease-treated DNA in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	10 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	1000 U (100 μl)

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 µM ATP, and 50% (v/v) Glycerol.
Format	Ribonuclease
Concentration	10 U/μl

Protocol	T4 Polynucleotide Kinase Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900074	E006	100µl	$73.85		Add to Cart

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