T4 Endonuclease V(T4 PDG) (1 U/uL)

Description
Review(s)

Mfr.No.

14542, 14542ES76, 14542ES80

Description

T4 Endonuclease V, also known as T4 PDG (Pyrimidine Dimer Glycosylase), is a bifunctional DNA glycosylase that combines the activities of DNA N-glycosylase and AP lyase. This enzyme is capable of recognizing and binding to cis-syn cyclobutane pyrimidine dimers, which are induced by ultraviolet radiation. It then cleaves the glycosidic bond at the 5' end of the pyrimidine dimers, releasing them and leaving an AP site. Subsequently, it utilizes its endonuclease activity to cleave the phosphodiester bond at the AP site, creating a one-nucleotide gap in the DNA strand.

Applications

DNA damage studies

Single cell gel electrophoresis (comet assay)

Specifications

Source	T4 Phage
Molecular Weight	16.1 KD
Concentration	1 U/?L
Unit Definition	One unit is defined as the amount of enzyme that catalyzes the conversion of 0.5 µg of UV irradiated supercoiled pUC19 DNA to > 95% nicked plasmid in a total reaction volume of 20 µL in 30 minutes at 37°C. Nicking is assessed by agarose gel electrophoresis. Irradiated plasmid contains an average of 3-5 pyrimidine dimers

Components

Components No.	Name	14542ES76	14542ES80
14542-A	T4 Endonuclease V(T4 PDG)(1 U/?L)	500 ?L	1 mL
14542-B	10 x T4 Endonuclease V(T4 PDG) Buffer	1 mL	2 mL

Shipping and Storage

This product should be stored at-25~-15? for 1 years.

Documents:

Safety Data Sheet

14542_MSDS_Ver.EN20250121.pdf

Manuals

14542_Manual _Ver.EN20250121.pdf

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHS20800186-1	14542ES76	500 U	$75		Add to Cart
BHS20800186-2	14542ES80	1000 U	$145		Add to Cart

T4 Endonuclease V(T4 PDG) (1 U/uL)

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