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Home / Enzymes, Inhibitors & Substrates

T4 DNA Polymerase

SKU : BHZ10900070

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E005
Description	T4 DNA Polymerase catalyzes the 5’→3’ synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3’→5’ exonuclease activity but lacks 5’→3’ exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3’-overhangs or 5’-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.
Description	Generates blunt end DNA by filling in 5'-overhangs or/and removing 3'-overhangs ; High fidelity due to strong 3’→5’ exonuclease activity ; Synthesis of labeled DNA probes by the replacement reaction ; Site-specific mutagenesis via primer extension from oligonucleotides
Notes	One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	5 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	500 U (100 μl)

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 100 mM KPO4 (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol.
Format	Polymerase
Concentration	5 U/μl

Protocol	T4 DNA Polymerase

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900070	E005	100µl	$101.67		Add to Cart

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