T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
Description
Cloning of restriction fragments ; Attaching double-stranded oligonucleotide linkers or adaptors to DNA ; Site-directed mutagenesis ; Nick repair of duplex DNA, RNA or DNA-RNA hybrids ; Self-circularization of linear DNA
Notes
This product is distributed for laboratory research only. Caution: Not for diagnostic use.