Restriction enzyme digestion of double-stranded DNA
Components
SmaI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked
Concentration
20000 U/ml
Heat Inactivation
65°C for 20 min
Quantity
10000 U (500 µl)
Recognition Site
Sequence CCC^GGG
Source
An E. coli strain that carries the cloned and modified SmaI gene from Serratia marcescens (ATCC 49779). The SmaI enzyme recognizes and cuts the sequence CCC^GGG.
Storage
-20°C
Shipping Condition
Ice Packs
Storage Condition
-20C
Buffer
Storage Buffer: 50 % Glycerol, 1 mM DTT, 10 mM Tris-HCl, 300 mM NaCl, 500 µg/ml BSA, 0.1 mM EDTA, pH 7.4 @ 25°C.Reaction Buffer: Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%