abm’s Site-Directed Mutagenesis Kit allows for efficient and rapid mutagenesis of double-stranded plasmid DNA. It enables the generation of point mutations, deletions and insertions at any site without design constraints. Site-directed mutagenesis is a useful tool to study changes in gene or protein function as well as easily make vector modifications.ProClone ™ Competent Cellsare recommended for use with this kit for the highest quality results.
Advantages:
Use of standard primers eliminates additional primer expenses – no phosphorylation step is required.
Ready-to-use 2X Bestaq™ MasterMix with high-fidelity DNA polymerase limits unwanted PCR-introduced errors.
PCR treatment reagents rapidly eliminate the template background without lengthy hands-on purification steps.
Pro Ligation-Free MasterMix results in highly efficient, seamless assembly of the mutant plasmid
Condensed 4 hour workflow, with less than 15 minutes of hands-on time for highly efficient and accurate mutagenesis.
Description
Generation of substitution, insertion or deletion mutations in double-stranded plasmid DNA.