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Home / Enzymes, Inhibitors & Substrates

RNase III, E.coli

SKU : BHZ10900097

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E020
Description	RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3´-overhangs with 5' phosphate and 3' hydroxyl termini.
Description	dsRNA digestion to short dsRNA fragments ; Useful for RNA processing, maturation and structure studies ; Directed cleavage of RNA ; siRNA preparation for RNA interference in mammalian cells ; Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Notes	One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	1 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	100 U (100 μl)

Storage	Store at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 50 mM Tris-HCl (pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
Format	Ribonuclease
Concentration	1 U/μl

Protocol	RNase III, E.coli Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900097	E020	100µl	$95.05		Add to Cart

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