RNase HII, Glycerol free (2 U/uL)

Mfr.No.

14541ES80, 14541ES92

Description

RNase HII is a ribonuclease inteal enzyme obtained from the Pyrococcus abyssi and recombinantly expressed in E. Coli. It recognizes the DNA-rN-DNA/DNA double strand, cutting at the site where ribonucleotides are incorporated into DNA. However, its activity on single-stranded RNA is very low, and it shows no cutting activity on dsDNA or ssDNA. RNase HII cuts at the site of a single ribonucleotide residue from the 5' end, producing a 5' phosphate group and a 3' hydroxyl end after cutting. This RNase HII enzyme has optimal activity at 70~75°C, is active between 50°C and 75°C, but has very low activity at room temperature. The product is highly thermostable, with almost no loss of activity after incubation at 95°C for 45 minutes, and is compatible with various PCR reaction systems. This product does not contain glycerol and is specifically used for the preparation of freeze-dried reagents.

Components

Name	14541ES80	14541ES92
RNase HII, Glycerol-free (2 U/μL)	500 μL	5× 1 mL

Notes

This product is for research use only.
Please operate with lab coats and disposable gloves, for your safety.

Applications Range	Detection by LAMP with high-sensitivity probes. RNase HII-dependent PCR (rhPCR). Removal of mismatched ribonucleotides formed during polymerase chain reaction. Degradation of the RNA portion of Okazaki fragments.

Storage	This product should be stored at 2～8℃ for 6 mouths.
Format	Ribonuclease

Protocals And Manuals	14541 _Manual

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHZ20800036-1	14541ES80	1 KU	$655		Add to Cart
BHZ20800036-2	14541ES92	10 KU	$5875		Add to Cart

RNase HII, Glycerol free (2 U/uL)

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