The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability.abm’s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs.
Description
Rapid multi-fragment cloning and assembly of whole vector constructs.
Clone in a promoter, gene, and tag, all in one step.
Rapid assembly of CRISPR multiplex gRNA vectors in one step.
Advantages Over Traditional Cloning
Rapidly assemble as many as six fragments into any vector in one step
Eliminates time-consuming multi-step traditional cloning methods.
Seamlessly clone your inserts anywhere in any vector.
Inserts are free of extra or unwanted base pairs.
Eliminates dependence on available restriction sites and compatible ends.