Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates.
Description
Rolling circle amplification (RCA) ; Multiple displacement amplification (MDA) ; Whole genome amplification (WGA) ; DNA template preparation for sequencing ; Protein-primed DNA amplification
Notes
One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
This product is distributed for laboratory research only. Caution: Not for diagnostic use .
Concentration
10 U/μl
Form
Enzyme supplied with 5X Reaction Buffer
Quantity
1000 U (100 μl)
Storage
Store all components at -20°C.
Shipping Condition
Dry Ice
Storage Condition
-20C
Buffer
Storage Buffer: 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5% Tween-20, 0.5% NP-40 and 50% (v/v) Glycerol.