PBCV DNA ligase (25 U/uL)

PBCV DNA Ligase, also known as SplintR ligase, PBCV-1 DNA Ligase, or Chlorella virus DNA ligase, is an ATP-dependent DNA ligase that efficiently catalyzes the ligation reaction of two adjacent DNA strands that are complementary to a single stranded RNA. In these two adjacent DNA strands, the one providing the 3' hydroxyl group is called the acceptor DNA strand, while the one providing the 5' phosphate group is called the donor DNA strand. During this ligation process, a complementary RNA strand acts as a "splint" or "scaffold" for the two DNA strands. The activity of PBCV DNA Ligase is much higher than that of traditional T4 DNA ligase and shows a strong affinity for DNA substrates with RNA as a splint (apparent Km = 1 nM, where a smaller Km value indicates a stronger affinity). It can detect unique RNA species at the subnanomolar level in complex mixtures, making it suitable for target enrichment processes in applications such as high-sensitivity RNA detection, next-generation sequencing, and molecular diagnostics.

Features

No residual nuclease, endonuclease, RNase, or phosphatase

Applications

Detection of microRNAs and other small RNAs based on probe method

Connecting single-stranded DNA through "clamp" fixation by complementary RNA sequences

Using DNA probes to achieve the detection of specific RNA

Detection of SNP or Alternative Splicing

RASL-seq analysis

Specifications

Components

Shipping and Storage

The products should be stored at -25? ~ -15? for 2 years.

Documents:

Safety Data Sheet

14962_MSDS_Ver.EN20241210.pdf

Manuals

14962_Manual_Ver.EN20241210.pdf