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Home / Enzymes, Inhibitors & Substrates

Nt.BstNBI (Bst Nickase)

SKU : BHZ10900095

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E042
Description	Nt.BstNBI (Bst Nickase) is a nicking endonuclease from Bacillus stearothermophilus that cleaves one DNA strand of a double-stranded DNA substrate. This site-specific endonuclease cleaves a single strand of DNA four base pairs away and 3’ of its recognition sequence.
Description	Single strand DNA nicking.
Notes	One unit is defined as the amount of Nt.BstNBI (Bst Nickase) that digests 1 µg of T7 DNA into 5 distinct bands (13, 9, 7, 6, and 4 kb) in 1 hour at 55°C in a reaction volume of 50 µl. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	10 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	1000 U (100 μl)

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 50 mM KCl, 200 µg/ml BSA and 50% (v/v) Glycerol.
Format	Endonuclease
Concentration	10 U/μl

Protocol	Nt.BstNBI (Bst Nickase) Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900095	E042	100µl	$76.50		Add to Cart

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