Inorganic pyrophosphatase, Thermostable catalyzes the hydrolysis reaction of inorganic pyrophosphate to orthophosphate.
Notes
One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
Concentration
2U/ul
Quantity
100 μl
Source
The source of this protein is a recombinant E. coli strain encoding pyrophosphatase from Thermococcus litoralis.
Shipping Condition
Dry Ice
Storage Condition
-20C
Buffer
Storage Buffer: 20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0 at 25°C.
Format
Phosphatase
Concentration
2U/ul
Reviews of Inorganic Pyrophosphatase, Thermostable