FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
Features
Rapid digestion: DNA was digested rapidly by enzyme for 5-15 min
Universal buffer: one tube reaction, simplifying the process
Stringent quality control
Applications
Molecular cloning
Southe blotting
SNP
Southe blotting
Genotyping
Specifications
Enzyme
DpnI
Cutting Site
5'-GAm6↓TC-3' 3'-CT↑Am6G-5'
Optimal Reaction Temperature
37℃
Sensitive to Heat Inactivation
YES
Type IIs RE
NO
Methylation Sensitivity
Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not EcoKI Methylation-Sensitive, EcoBI Methylation-Sensitive
Star Activity
3 h incubation did not show star activity, and delayed enzyme digestion might show star activity
Isoschizomers
NO
Components
Components No.
Name
15052ES50 (50 T)
15052-A
FuniCut™ DpnI
50 μL
15052-B
10× FuniCut™ Buffer
1 mL
15052-C
10× FuniCut™ Color Buffer
1 mL
Storage
The product should be stored at -25℃ ~ -15℃ for two years. Please avoid repeated freeze-thaw.