FuniCut enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut enzymes show superior activity in the universal FuniCut Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
Features
Rapid digestion: DNA was digested rapidly by enzyme for 5-15 min
Universal buffer: one tube reaction, simplifying the process
Stringent quality control
Applications
Molecular cloning
Southern blotting
SNP
Southern blotting
Genotyping
Specifications
Enzyme
DpnI
Cutting Site
5'-GAm6?TC-3'3'-CT?Am6G-5'
Optimal Reaction Temperature
37?
Sensitive to Heat Inactivation
YES
Type IIs RE
NO
Methylation Sensitivity
Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not EcoKI Methylation-Sensitive, EcoBI Methylation-Sensitive
Star Activity
3 h incubation did not show star activity, and delayed enzyme digestion might show star activity
Isoschizomers
NO
Components
Components No.
Name
15052ES50 (50 T)
15052-A
FuniCut DpnI
50 ?L
15052-B
10× FuniCut Buffer
1 mL
15052-C
10× FuniCut Color Buffer
1 mL
Storage
The product should be stored at -25? ~ -15? for two years. Please avoid repeated freeze-thaw.