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Home / Enzymes, Inhibitors & Substrates

Exonuclease I, E. coli

SKU : BHZ10900065

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E015
Description	Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5’ direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3’-terminus is phosphorylated.
Description	Removal of single-stranded primer oligonucleotides from: ; o PCR mixtures for applications involving sequencing or labelling ; o from nucleic acid mixtures ; Assaying for the presence of single-stranded DNA with a 3'-hydroxyl terminus ; Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Notes	One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	20 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	2000 U (100 μl)

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 20 mM HEPES (pH 7.1), 150 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% (v/v) Glycerol.
Format	Endonuclease
Concentration	20 U/μl

Protocol	Exonuclease I Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900065	E015	100µl	$57.95		Add to Cart

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