Exonuclease I (20 U/uL)

Exonuclease I, sourced from a genetically engineered E. coli strain expressing the Exo I gene, exhibits exonuclease activity that digests single-stranded DNA from the 3' to 5' end. It sequentially releases deoxyribonucleotide 5'-monophosphates, preserving the integrity of the 5'-terminal dinucleotides. This enzyme is predominantly utilized for the breakdown and removal of primers following PCR amplification. It remains inactive towards double-stranded DNA and DNA strands with 3' hydroxyl ends that are blocked by phosphorylation or acetylation.

Features

No residual exonuclease (double-stranded), endonuclease, or RNase

Inactivated by simply treating it at 80°C for 15 minutes

Applications

Remove single-stranded primers from the PCR reaction system before Sanger DNA sequencing or SNP analysis

Remove single-stranded primers from the nested PCR reaction system

Eliminate linear single-stranded DNA from the sample, leaving behind double-stranded DNA

Specifications