Endonuclease VIII (10 U/uL)

Endonuclease VIII is a DNA repair enzyme that possesses both N-glycosylase activity and AP-lyase activity. The N-glycosylase activity can release damaged pyrimidine bases from double-stranded DNA, creating an abasic (AP) site. The AP-lyase activity then cleaves the phosphodiester bond at the AP site, resulting in a gap with a 5' phosphate and a 3' phosphate. The damaged bases that Endonuclease VIII recognizes and excises include: urea, 5,6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methoxyhydantoin, uracil glycol, 6-hydroxy-5,6-dihydrothymidine, and methylhydroxypropanone.

Features

No residual of exonucleases, endonucleases, or RNases

High-efficiency removal of damaged bases

Applications

Single-cell gel electrophoresis (Comet assay)

Alkaline elution

Alkaline unwinding

Specifications

 Components

Shipping and Storage

The products should be stored at -25? ~ -15? for 1 year.

Figures

Figure1. Base excision repair effect comparison

Note: 20 pmol of oligonucleotides were catalyzed by Endonuclease VIII from Yeasen and SupplierA, respectively, in a 20 µL reaction system with the addition of 5~40 U of Endonuclease VIII and equivalent enzyme activity of UDG (Cat#10303ES), and incubated at 37? for 1 hour. The results of agarose gel electrophoresis showed that the base excision effects of Yeasen 's Endonuclease VIII and Supplier A's were consistent.

Documents:

Safety Data Sheet

14536_MSDS_Ver.EN20241210.pdf

Manuals

14536_Manual_Ver.EN20241210.pdf