Endonuclease IV(10 U/uL)

Description
Review(s)

Mfr.No.

14543, 14543ES80, 14543ES90

Description

Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5´ to the lesion leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´ terminus. The enzyme also has a 3´ -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5-phosphate and phosphate from the 3´ end of DNA.

Applications

Single cell gel electrophoresis (Comet assay)

Alkaline elution

Alkaline unwinding

Specifications

Source	E.coli
Molecular Weight	32.5 KD
Concentration	10 U/?L
Unit Definition	One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34-mer oligonucleotide duplex containing a single AP site in a total reaction volume of 10 µl in 1 hour at 37°C. An AP site is created by treating 10 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 minutes at 37°C.

Components

Components No.	Name	14543ES80	14543ES90
14543-A	Endonuclease IV(10 U/?L)	100 ?L	500 ?L
14543-B	10 x Endonuclease IV Buffer	0.8 mL	1.25 mL

Shipping and Storage

This product should be stored at-25~-15? for 1 years.

Documents:

Safety Data Sheet

14543_MSDS_Ver.EN20250121.pdf

Manuals

14543_Manual _Ver.EN20250121.pdf

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHS20800185-1	14543ES80	1000 U	$105		Add to Cart
BHS20800185-2	14543ES90	5000 U	$435		Add to Cart

Endonuclease IV(10 U/uL)

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