Endo H

Mfr.No.

20414ES92, 20414ES97

Description

Endo H is a recombinant glycosidase that can cleave the core structure of high-mannose and some hybrid oligosaccharides in N-glycoproteins, removing the N-linked high-mannose from glycoproteins. Endo H is cloned from the corrugated actinomycete (Streptomyces plicatus) and recombinantly expressed in yeast. This product is tagged with His and is commonly used for complete deglycosylation of antibodies and related proteins.

Feature

High purity—No protease, glycosidase contamination, purity ≥95%.
High Activity— 1,000,000 U/mL.
Good compatibility—Can be used denaturing or no-denaturing conditions.

Application

Glycan Sequencing
Proteomics
Glycoprotein Analysis
Recombinant Glycoprotein Expression

Specification

English synonym	Endo H
Source	Yeast recombinant expression
Molecular Weight	60 kDa
Specific Activity	1,000,000 U/mL
Storage buffer	20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA
Unit Definition	One unit of endoglycosidase H is defined as the amount of enzyme required to hydrolyze >95% of the oligomannose from 10 μg of denatured RNase B in 1 hour at 37°C in a 10 μL reaction volume.

Components

Components No.	Name	20414ES92	20414ES97
20414-A	Endo H	10000 U	50000 U
20414-B1	Buffer 1 (10×)	100 μL	500 μL
20414-B2	Buffer 2 (10×)	200 μL	1000 μL

Storage	The product can be stored at -25 to -15℃ for one year.
Format	Glycosylase

Protocals And Manuals	Manual

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHZ20800010-1	20414ES92	10000 U	$70		Add to Cart
BHZ20800010-2	20414ES97	50000 U	$290		Add to Cart

Endo H

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