E.coli poly(A) Polymerase

E.coli Poly(A) polymerase, also known as Poly A tail-adding enzyme, catalyzes the addition of AMP converted from ATP to the 3 'end of single-stranded RNA in A template-independent manner to form Poly(A) tail. E.coli Poly(A) polymerase can use a variety of single-stranded RNA as a substrate, but DNA can not be used as a substrate. Double-stranded RNA and short oligonucleotides are not recommended as substrates. The Poly(A) tailing reaction catalyzed by E.coli Poly(A) Polymerase can only use ATP, but not ADP or dATP. The incorporation of CTP and UTP was less than 5% of that of ATP. When GTP is used, it cannot be added to the 3 'end of the RNA at all.

Features

When applied to miRNA detection, it demonstrates enhanced sensitivity.

No residual nuclease or RNase.

Applications

RNA Modification.

miRNA Expression Analysis.

cDNA Synthesis & Reverse Transcriptases Products.

RNA Synthesis In vitro Transcription (IVT).

Specifications

Components

Shipping and Storage

This product should be stored at -25 ~ -15^oC for 2 years.

Documents:      

Safety Data Sheet

14801_MSDS_Ver.EN20250208.pdf

Manuals

14801_Manual_Ver.EN20231009.pdf