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Home / Enzymes, Inhibitors & Substrates

E. coli DNA Polymerase I

SKU : BHZ10900075

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Applied Biological Materials (abm) Inc.

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Mfr.No.	G469
Description	E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3’→5’ and 5’→3’ exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing chain via 5´→3´ exonuclease activity.
Description	Generation of labeled DNA probes by nick translation ; Second-strand synthesis of cDNA ; Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Notes	One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	5 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	500 U (100 μl)

Storage	Store all components at -20°C.
Shipping Condition	Ice Packs
Storage Condition	-20C
Buffer	Storage Buffer: 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
Format	Polymerase
Concentration	5 U/μl

Protocol	E. coli DNA Polymerase I Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900075	G469	100µl	$48.25		Add to Cart

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