Deoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.
Description
Removes DNA from protein preparations and RNA samples ; Mediates nick translation ; Generates random fragments for dideoxy sequencing ; Degrade template DNA following in vitro transcription ; Mediate DNase I foot-printing
Notes
One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.
This product is distributed for laboratory research onlyCaution: Not for diagnostic use.