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Home / Enzymes, Inhibitors & Substrates

DNA Polymerase I Large (Klenow) Fragment

SKU : BHZ10900033

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E013
Description	DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I . The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.
Description	DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs ; cDNA second-strand synthesis ; Generate single-stranded DNA probes using random primers ; Site-directed DNA mutagenesis using synthetic oligonucleotides ; Dideoxy DNA sequencing of single- or double-stranded DNA templates ; 3’→5’ exonuclease activity can blunt a 3’-overhang
Notes	One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Concentration	5 U/μl
Form	Enzyme supplied with 10X Reaction Buffer
Quantity	500 U (100 μl)

Storage	Store all components at -20°C.
Shipping Condition	Dry Ice
Storage Condition	-20C
Buffer	Storage Buffer: 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
Format	Polymerase
Concentration	5 U/μl

Protocol	Large Klenow Fragment Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900033	E013	100µl	$73.85		Add to Cart

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