CleaScrip^TM T7 RNA Polymerase (GMP Grade,low dsRNA, 250 U/uL)

Description
Review(s)

Mfr.No.

10629, 10629ES10, 10629ES60, 10629ES72

Description

This is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'?3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.

Note: G* is the first base of the RNA transcript.

Feature

dsRNA level lower to about 1/100000
Comptiable with Trilink CleanCap AG, LZCap
High yields comparable to WT
Lower Cap input
Animal origin-free (AOF)

Please find information on the development of this enzyme.

Components

Components No.	Name	10629ES10	10629ES60	10629ES72	10629ES86
		(10 KU)	(100 KU)	(250 KU)	(2,500 KU)
10629	CleaScrip T7 RNA Polymerase (GMP Grrade, low dsRNA, 250 U/?L)	40 ?L	400 ?L	1 mL	10 mL

Specifications

Source	Recombinant E. coli with T7 RNA Polymerase gene
Optimum Temperature	37?
Storage Buffer	50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100?50% (v/v) glycerin?pH7.9 at 25?
Unit Definition	The amount of enzyme required to incorporate 1 nmol of [³H

Have you used this product?

Rate it now..!!

Combination SKU	Supplier No.	Size	Your price
BHS20800028-1	10629ES10	10 KU	$198	Add to Cart
BHS20800028-2	10629ES60	100 KU	$1675	Add to Cart
BHS20800028-3	10629ES72	250 KU	$3775	Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.