Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive
Concentration
20000 U/ml
Heat Inactivation
65°C for 20 min
Quantity
1500 U (75 µl)
Recognition Site
Sequence GAAGAC(N2)^
Source
An E. coli strain that carries the cloned and modified BbsI gene from Bacillus laterosporus (C. Story). The BbsI enzyme recognizes and cuts the sequence GAAGAC(N2)^.
Storage
-80°C
Shipping Condition
Ice Packs
Storage Condition
-20C
Buffer
Storage Buffer: 0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 300 mM KCl, 300 µg/ml BSA, pH 7.4 @ 25 °C.Reaction Buffer: Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%