Mouse TSH(Thyroid Stimulating Hormone) CLIA Kit

Mfr.No.

E-CL-M0645

Antigen

TSH

Alternative Names

Thyrotropin

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
20	32059	35905	33982	33950
10	11235	13705	12470	12438
5	5410	5090	5250	5218
2.5	2487	2561	2524	2492
1.25	1492	1274	1383	1351
0.625	899	835	867	835
0.31	583	663	623	591
0	31	33	32	--

Reactivity

Mouse

Specificity

This kit recognizes Mouse TSH in samples. No significant cross-reactivity or interference between Mouse TSH and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

0.313--20 ng/mL

Linearity

Samples were spiked with high concentrations of Mouse TSH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	98-113	95-107	88-99
1:2	Average (%)	104	102	94
1:4	Range (%)	103-115	96-108	101-117
1:4	Average (%)	109	102	107
1:8	Range (%)	96-111	103-115	91-105
1:8	Average (%)	103	109	97
1:16	Range (%)	99-114	93-108	93-108
1:16	Average (%)	105	99	100

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TSH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TSH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.06	2.51	9.52	1.08	2.50	9.91
Standard deviation	0.10	0.19	0.91	0.12	0.21	0.85
C V (%)	9.43	7.57	9.56	11.11	8.40	8.58

Recovery

The recovery of Mouse TSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	101-112	106
EDTA plasma (n=5)	87-101	94
Cell culture media (n=5)	97-110	103

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.19 ng/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10301831	E-CL-M0645	96T	$682	Add to Cart
BHE10301831	E-CL-M0645	96T*5	$3069	Add to Cart
BHE10301831	E-CL-M0645	96T*10	$5797	Add to Cart
BHE10301831	E-CL-M0645	48T	$545.60	Add to Cart

Mouse TSH(Thyroid Stimulating Hormone) CLIA Kit

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