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Home / Assay Kits / ELISA Kits

ELISA Kit for Rat Glyceraldehyde-3-phosphate dehydrogenase

SKU : BHE13803829

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1932r
Alternative Names	38 kDa BFA-dependent ADP-ribosylation substrate ; BARS-38 ; Peptidyl-cysteine S-nitrosylase GAPDH ; 2.6.99.- ; Gapd
Categories	Elisa
Function	Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Specificity	Natural and recombinant Rat Glyceraldehyde-3-phosphate dehydrogenase
Subcellular Location	Cytoplasm cytosol Nucleus Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal.
Detection Range	0.156-10ng/mL
Entryname	G3P_RAT
Method Type	Sandwich
Platform	PF02800
Protein Name	Glyceraldehyde-3-phosphate dehydrogenase
Sensitivity	0.065ng/mL
Sequence	50: MVKVGVNGFG ; RIGRLVTRAA ; FSCDKVDIVA ; INDPFIDLNY ; MVYMFQYDST ; 100: HGKFNGTVKA ; ENGKLVINGK ; PITIFQERDP ; ANIKWGDAGA ; EYVVESTGVF ; 150: TTMEKAGAHL ; KGGAKRVIIS ; APSADAPMFV ; MGVNHEKYDN ; SLKIVSNASC ; 200: TTNCLAPLAK ; VIHDNFGIVE ; GLMTTVHAIT ; ATQKTVDGPS ; GKLWRDGRGA ; 250: AQNIIPASTG ; AAKAVGKVIP ; ELNGKLTGMA ; FRVPTPNVSV ; VDLTCRLEKP ; 300: AKYDDIKKVV ; KQAAEGPLKG ; ILGYTEDQVV ; SCDFNSNSHS ; STFDAGAGIA ; 333: LNDNFVKLIS ; WYDNEYGYSN ; RVVDLMAYMA ; SKE
Sequence Length	333
String	P04797
Sub Unit	Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct (By similarity). Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation.

Uniprot	P04797
Gene Name	Gapdh
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:24383
Unigene	Rn.129558

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SKU	Supplier No.	Size	Your price	Quantity
BHE13803829	E1932r	96T	$796		Add to Cart

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