ELISA Kit for Mouse Zinc finger protein 335

Mfr.No.

E1019m

Alternative Names

NRC-interacting factor 1 ; NIF-1 ; Zfp335

Categories

Elisa

Function

Component or associated component of some histone methyltransferase complexes may regulate transcription through recruitment of those complexes on gene promoters. Enhances ligand-dependent transcriptional activation by nuclear hormone receptors. Plays an important role in neural progenitor cell proliferation and self-renewal through the regulation of specific genes involved brain development, including REST. Also controls the expression of genes involved in somatic development and regulates, for instance, lymphoblast proliferation.

Specificity

Natural and recombinant Mouse Zinc finger protein 335

Subcellular Location

Nucleus

Detection Range

0.312-20ng/ml

Entryname

ZN335_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-105%	93-101%	95-105%	92-106%
EDTA plasma(n=5)	98-105%	94-105%	97-106%	94-106%
heparin plasma(n=5)	96-104%	97-110%	96-111%	96-109%

Method Type

Sandwich

Platform

PF00096

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Protein Name

Zinc finger protein 335

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	94-103
Plasma	98	95-106

Sensitivity

0.158ng/mL

Sequence

50:

MEENEVESSS ; DAAPRPGQPE ; EPSESGLGVC ; TSEAVSADSS ; DAATVPGLTE ;

100:

ADDSGVGQSS ; DGGNHSVEEV ; SESISTDPLP ; HGCLPDSSSV ; SRGPVAEMPG ;

150:

GPPALVHSSV ; LPDPSMLVSD ; CTASSSDLGS ; AIDKIIESTI ; GPDLIQSCIT ;

200:

VTSGEEGGAE ; TTQYLILQGP ; DDGAPMASSM ; STSTLANSLA ; AIEALADGPT ;

250:

STSACLEPPE ; EPQGDPSSVA ; QQPPAPVTEE ; LDLQSLEAMM ; EVVVVQQFKC ;

300:

KMCQYRSSTK ; ATLLRHMRER ; HFRPALAAAA ; AATGKRGRVR ; KWGTSTKTTE ;

350:

EDRPEEEEED ; DDIVDAGAID ; DLEEDSDYNP ; AEDEPRGRQL ; RLQRPTPSTP ;

400:

RPRRRPGRPR ; KLPRLETSDL ; HDGVGQPLVS ; SQSTQSPPEL ; QDLEAPSSSG ;

450:

LRALGKVGRG ; LVESGVSQSD ; AENAAPSCQD ; EADAPPRRRG ; RPSRRFLGKK ;

500:

YRKYYYKSPK ; PLLRPYLCRI ; CGSRFLSHED ; LRFHVNSHEA ; GDPQLFRCLQ ;

550:

CSYRSRRWSS ; LKEHMFNHVG ; SKPYKCDECS ; YTSVYRKDVI ; RHAAVHSQDR ;

600:

KKRPDPTPKL ; SSFPCPVCGR ; VYPMQKRLTQ ; HMKTHSTEKP ; HMCDKCGKSF ;

650:

KKRYTFKMHL ; LTHIQAVANR ; RFKCEFCEFV ; CEDKKALLNH ; QLSHVSDKPF ;

700:

KCSFCPYRTF ; REDFLLSHVA ; VKHTGAKPFA ; CEYCHFSTRH ; KKNLRLHVRC ;

750:

RHANSFEEWG ; RRHPEEPPSR ; RRPFFSLQQI ; EELKQQHSTA ; PGPPLSSPGP ;

800:

EAPQEPAPFQ ; SPETPPLLCP ; DALGGTTIIY ; QQGAEESTAV ; ATQTALDLLL ;

850:

NMSAQRELGA ; TALQVAVVKS ; EGIEAELTST ; GGQPSPEDTT ; PRVVTLHMAE ;

900:

SGSSVAAESQ ; LGPSDLQQIA ; LPSGPFGGAS ; YSVITAPPVE ; GRTSASGPPY ;

950:

REEPPGEAAQ ; AVVVSDTLKE ; AGTHYIMAAD ; GTQLHHIELT ; ADGSISFPSP ;

1000:

DTLAPGTKWP ; LLQCGGPPRD ; GSEVLSPTKT ; HHMGGSQGSS ; TPPPAASHTL ;

1050:

GLVVPQSPPS ; AAASSTKKFS ; CKVCSEAFPS ; RAEMESHKRA ; HAGPAAFKCP ;

1100:

DCPFSARQWP ; EVRAHMAQHS ; SLRPHQCNQC ; SFASKNKKDL ; RRHMLTHTNE ;

1150:

KPFSCHVCGQ ; RFNRNGHLKF ; HIQRLHSIDG ; RKTGTSTARA ; PAQTIILNSE ;

1200:

EETLATLHTA ; FQSSHGVLGT ; ERLQQALSQE ; HIIVAQEQTV ; TNQEEATYIQ ;

1250:

EITADGQTVQ ; HLVTSDNQVQ ; YIISQDGVQH ; LLPQEYVVVP ; DGHHIQVQEG ;

1300:

QITHIQYEQG ; TPFLQESQIQ ; YVPVSPSQQL ; VTQAQLEAAA ; HSAVTAVADA ;

1337:

AMAQAQGLFG ; TEEAVPEQIH ; QLQHQGIEYD ; VITLSDD

Sequence Length

1337

Sub Unit

Interacts with NCOA6; may enhance ligand-dependent transcriptional activation by nuclear hormone receptors (By similarity). Interacts with CNOT6 (By similarity). Interacts with RQCD1; the interaction is direct (By similarity). Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity (By similarity). Interacts with members of histone H3'Lys4'(H3K4) methyltransferase complexes ASCL2, CXXC1, KMT2A/MLL1, SETD1A (By similarity). Interacts with RBBP5 and WDR5.

Uniprot	A2A5K6
Gene Name	Znf335
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:329559
Unigene	Mm.330776

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ELISA Kit for Mouse Zinc finger protein 335

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