ELISA Kit for Mouse Multidrug resistance protein 2

Mfr.No.

E10409m

Alternative Names

ATP-binding cassette sub-family B member 4 ; P-glycoprotein 2 ; Mdr2 ; Pgy-2 ; Pgy2

Categories

Elisa

Function

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Mouse MDR2 is not capable of conferring drug resistance.

Specificity

Natural and recombinant Mouse Multidrug resistance protein 2

Subcellular Location

Membrane Multi-pass membrane protein

Detection Range

0.156-10ng/mL

Entryname

MDR2_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ABCB4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-105%	95-103%	96-105%	92-109%
EDTA plasma(n=5)	93-105%	92-107%	95-103%	93-106%
heparin plasma(n=5)	94-104%	91-105%	92-108%	91-109%

Method Type

Sandwich

Platform

PF00664

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.7%

Protein Name

Multidrug resistance protein 2

Recovery

Recovery was determined by spiking various levels of ABCB4 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	97-108
Plasma	99	95-106

Sensitivity

0.088ng/mL

Sequence

50:

MDLEAARNGT ; ARRLDGDFEL ; GSISNQGREK ; KKKVNLIGLL ; TLFRYSDWQD ;

100:

KLFMFLGTLM ; AIAHGSGLPL ; MMIVFGEMTD ; KFVDNTGNFS ; LPVNFSLSML ;

150:

NPGRILEEEM ; TRYAYYYSGL ; GGGVLVAAYI ; QVSFWTLAAG ; RQIKKIRQKF ;

200:

FHAILRQEMG ; WFDIKGTTEL ; NTRLTDDVSK ; ISEGIGDKVG ; MFFQAIATFF ;

250:

AGFIVGFIRG ; WKLTLVIMAI ; SPILGLSTAV ; WAKILSTFSD ; KELAAYAKAG ;

300:

AVAEEALGAI ; RTVIAFGGQN ; KELERYQKHL ; ENAKKIGIKK ; AISANISMGI ;

350:

AFLLIYASYA ; LAFWYGSTLV ; ISKEYTIGNA ; MTVFFSILIG ; AFSVGQAAPC ;

400:

IDAFANARGA ; AYVIFDIIDN ; NPKIDSFSER ; GHKPDNIKGN ; LEFSDVHFSY ;

450:

PSRANIKILK ; GLNLKVKSGQ ; TVALVGNSGC ; GKSTTVQLLQ ; RLYDPTEGKI ;

500:

SIDGQDIRNF ; NVRCLREIIG ; VVSQEPVLFS ; TTIAENIRYG ; RGNVTMDEIE ;

550:

KAVKEANAYD ; FIMKLPQKFD ; TLVGDRGAQL ; SGGQKQRIAI ; ARALVRNPKI ;

600:

LLLDEATSAL ; DTESEAEVQA ; ALDKAREGRT ; TIVIAHRLST ; IRNADVIAGF ;

650:

EDGVIVEQGS ; HSELMKKEGI ; YFRLVNMQTA ; GSQILSEEFE ; VELSDEKAAG ;

700:

DVAPNGWKAR ; IFRNSTKKSL ; KSPHQNRLDE ; ETNELDANVP ; PVSFLKVLKL ;

750:

NKTEWPYFVV ; GTVCAIANGA ; LQPAFSIILS ; EMIAIFGPGD ; DAVKQQKCNM ;

800:

FSLVFLGLGV ; LSFFTFFLQG ; FTFGKAGEIL ; TTRLRSMAFK ; AMLRQDMSWF ;

850:

DDHKNSTGAL ; STRLATDAAQ ; VQGATGTRLA ; LIAQNTANLG ; TGIIISFIYG ;

900:

WQLTLLLLSV ; VPFIAVAGIV ; EMKMLAGNAK ; RDKKEMEAAG ; KIATEAIENI ;

950:

RTVVSLTQER ; KFESMYVEKL ; HGPYRNSVRK ; AHIYGITFSI ; SQAFMYFSYA ;

1000:

GCFRFGSYLI ; VNGHMRFKDV ; ILVFSAIVLG ; AVALGHASSF ; APDYAKAKLS ;

1050:

AAYLFSLFER ; QPLIDSYSGE ; GLWPDKFEGS ; VTFNEVVFNY ; PTRANVPVLQ ;

1100:

GLSLEVKKGQ ; TLALVGSSGC ; GKSTVVQLLE ; RFYDPMAGSV ; LLDGQEAKKL ;

1150:

NVQWLRAQLG ; IVSQEPILFD ; CSIAENIAYG ; DNSRVVPHDE ; IVRAAKEANI ;

1200:

HPFIETLPQK ; YNTRVGDKGT ; QLSGGQKQRI ; AIARALIRQP ; RVLLLDEATS ;

1250:

ALDTESEKVV ; QEALDKAREG ; RTCIVIAHRL ; STIQNADLIV ; VIENGKVKEH ;

1276:

GTHQQLLAQK ; GIYFSMVNIQ ; AGTQNL

Sequence Length

1276

String

P21440

Sub Unit

Interacts with HAX1.

Uniprot	P21440
Gene Name	Abcb4
Immunogen Species	Mus musculus (Mouse)
Unigene	Mm.297825

Reference

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ELISA Kit for Mouse Multidrug resistance protein 2

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