ELISA Kit for Mouse Dickkopf-related protein 4

Mfr.No.

E5890m

Categories

Elisa

Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disease.

Specificity

Natural and recombinant Mouse Dickkopf-related protein 4

Subcellular Location

Secreted

Entryname

DKK4_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF04706

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Dickkopf-related protein 4

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sequence

50:

MVLVTLLGLS ; WFCSPLAALV ; LDFNNIKSSA ; DVQGAGKGSL ; CASDRDCSEG ;

100:

KFCLAFHDER ; SFCATCRRVR ; RRCQRSAVCC ; PGTVCVNDVC ; TAVEDTRPVM ;

150:

DRNTDGQDGA ; YAEGTTKWPA ; EENRPQGKPS ; TKKSQSSKGQ ; EGESCLRTSD ;

200:

CGPGLCCARH ; FWTKICKPVL ; REGQVCSRRG ; HKDTAQAPEI ; FQRCDCGPGL ;

221:

TCRSQVTSNR ; QHSRLRVCQR ; I

Sequence Length

221

String

Q8VEJ3

Sub Unit

Interacts with LRP5 and LRP6.

Uniprot	Q8VEJ3
Gene Name	Dkk4
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:234130
Unigene	Mm.157322

Reference	1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Czech II. tissue: Mammary tumor. 2. "Function and biological roles of the Dickkopf family of Wnt modulators." Niehrs C. Oncogene25:7469-7481(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW OF THE DKK FAMILY

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SKU	Supplier No.	Size	Your price	Quantity
BHE13803933	E5890m	96T	$776		Add to Cart

ELISA Kit for Mouse Dickkopf-related protein 4

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