ELISA Kit for Mouse Bactericidal permeability-increasing protein

Mfr.No.

E1234m

Categories

Elisa

Function

The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.

Specificity

Natural and recombinant Mouse Bactericidal permeability-increasing protein

Subcellular Location

Secreted Cytoplasmic granule membrane Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.

Detection Range

78-5000pg/mL

Entryname

BPI_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BPI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	80-92%	94-105%	96-103%	95-108%
EDTA plasma(n=5)	91-102%	96-103%	89-95%	86-102%
heparin plasma(n=5)	84-98%	85-99%	79-91%	96-101%

Method Type

Sandwich

Platform

PF01273

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.2

Protein Name

Bactericidal permeability-increasing protein

Recovery

Recovery was determined by spiking various levels of BPI into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	99	91-108
Plasma	95	89-97

Sensitivity

39.3pg/mL

Sequence

50:

MTWAPDNVRK ; WSALLLLAII ; GTALTAATDP ; GFVAMISQKG ; LDFACQQGVV ;

100:

ELQKELQAIS ; VPDFSGVFKI ; KHLGKGSYEF ; YSMAVDGFHI ; PNPKIEMLPS ;

150:

DGLRVFIKDA ; SIKINGKWMS ; RKNFLKAGGN ; FELSIQGVSI ; STDLILGSDS ;

200:

SGHITTICSN ; CDSHIDSVHI ; KISGSMLGWL ; IRLFHRKIET ; SLKNIIYKKI ;

250:

CKIVRDSVSS ; KLQPYLKTLS ; VITRVDDVTS ; VDYSLLAPLT ; TTNQFLEGQL ;

300:

KGEFFWRGHR ; DPLPIHPPVM ; RFVPNGAYMV ; CMGISDYFFN ; TEVLAYQQSG ;

350:

TLKMTLGGQL ; LSNNGRFQLN ; TDFLRTFLPK ; VAKMFPSMGV ; QLLISAPVPV ;

400:

HLSIQPSGLS ; FNPKLETQAF ; VVLPNASLVP ; LFVLGMKTNA ; SLEVDAEENR ;

450:

LVGEMKLGSR ; WLLELKESKF ; GPFKVEYLED ; VINYLVSTLV ; LPKINERLRR ;

483:

GFPLPLPAGI ; RFSHFTFYPY ; QNFLLLEADL ; HLI

Sequence Length

483

String

Q67E05

Sub Unit

Monomer. Homodimer; disulfide-linked.

Uniprot	Q67E05
Gene Name	Bpi
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:329547
Unigene	Mm.260883

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SKU	Supplier No.	Size	Your price	Quantity
BHE13801827	E1234m	96T	$776		Add to Cart

ELISA Kit for Mouse Bactericidal permeability-increasing protein

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