Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors.
Specificity
Natural and recombinant Human Glutathione peroxidase 2
Subcellular Location
Cytoplasm Mainly cytoplasmic.
Detection Range
78-5000pg/mL
Entryname
GPX2_HUMAN
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gpx2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤7.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤12.0%
Protein Name
Glutathione peroxidase 2
Recovery
Recovery was determined by spiking various levels of Gpx2 into serum and plasma .
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4.
NIEHS SNPs program Submitted (2004-10) to the EMBL/GenBank/DDBJ databases
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8.
"Crystal structure of the selenocysteine to cysteine mutant of human glutathione peroxidase 2 (GPX2)." Structural genomics consortium (SGC) Submitted (2009-02) to the PDB data bank
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Reviews of ELISA Kit for Human Glutathione peroxidase 2