The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. Regulates the alteative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Specificity
Natural and recombinant Human DNA topoisomerase 1
Subcellular Location
Nucleus nucleolus Nucleus nucleoplasm Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.