DENARASE® ELISA Kit

Mfr.No.

41901-1, 41901-2

Description

Best in class performance:
The carefully selected monoclonal antibodies provide an unmatched sensitivity and reproducibility in comparison to similar endonuclease ELISA Kits that work with polyclonal antibodies.

Best sensitivity:
Endonuclease concentrations in final pharmaceutical product are often below the limit of detection of previous state-of-the-art kits. These values may raise uncertainty, as low signals as a result of material failure or human error cannot be excluded. However, with the DENARASE® ELISA Kit you can detect and quantify up to 30 times lower endonuclease concentrations compared to competitor ELISA Kits. This makes the assay extremely reliable, even when expected concentrations are at a very low level.

Best reproducibility:
The highly specific monoclonal antibodies provide excellent reproducible results in both inter and intra assays. This enables an easy uptake in routine applications and avoids repeats in case of unclear results.

Broad working range:
The broad working range of the Kit enables a convenient handling of samples as over-dilution is unlikely to happen.

Save time:

Save up to 50% time compared to competitor kits.

Featured

Highest sensitivity
• Limit of Detection = 4 pg/mL
• Limit of Quantification = 12 pg/mL
• Reliably detect and quantify very low endonuclease levels in your final product
• Test the performance of individual DSP operations

Best reproducibility
(inter and intra assay, see also Prevalidation Report)
• Avoids repeats because of unclear results
• Easy uptake in routine applications
• Excellent linearity of dilution

50% Shorter assay time
• Savings on manpower and equipment occupation

Broad working range
at low concentration
• More comfort with dilution of samples to meet the range
• Less risk of over-diluting the samples

Size

The kit consists of a precoated micro test plate and ready to use reagents
The capacity of 1 kit is 480 measurements

Introduction

Kit Contents

No.	Reagent	Details	Quantity
1	Microtest plate (ready-to-use)	96 wells (12 strips of 8 wells), pre-coated with a monoclonal antibody specific for Serratia marcescens endonuclease	5 microplates
2	Washing & dilution buffer (10x)	Tris buffered saline containing surfactant and preservative, 10x concentrate	3 x 100 mL
3	Washing & dilution buffer (10x)	Tris buffered saline containing surfactant and preservative, 10x concentrate	1 x 20 mL
4	DENARASE Standard (10 µg/mL)	DENARASE endonuclease in a buffered solution with preservative	1 x 0.1 mL
5	Detector antibody (100x)	Monoclonal antibody specific for Serratia marcescens endonuclease, conjugated to biotin, in a buffered solution with preservative, 100x concentrate	1 x 0.75 mL
6	Enzyme conjugate (100x)	Streptavidin-conjugated horseradish peroxidase in stabilized solution, 100x concentrate	1 x 0.75 mL
7	Substrate solution	TMB One Substrate solution, ready-to-use	1 x 75 mL
8	Stop solution	0.5 M sulfuric acid	1 x 75 mL

Reference	1. Kawka, Karina, et al. "Integrated development of enzymatic DNA digestion and membrane chromatography processes for the purification of therapeutic adenoviruses." Separation and Purification Technology 254 (2021): 117503. 2. Reiter, Katrin, et al. "Separation of influenza virus‐like particles from baculovirus by polymer‐grafted anion exchanger." Journal of separation science 43.12 (2020): 2270-2278. 3. Ruiz-Sorribas, Albert, et al. "Hydrolytic Enzymes as Potentiators of Antimicrobials against an Inter-Kingdom Biofilm Model." Microbiology spectrum 10.1 (2022): e02589-21. 4. Lerer, Elad, et al. "Highly Efficient Purification of Recombinant VSV-∆ G-Spike Vaccine against SARS-CoV-2 by Flow-Through Chromatography." BioTech 10.4 (2021): 22. 5. Düring, Daniel N., et al. "Fast retrograde access to projection neuron circuits underlying vocal learning in songbirds." Cell reports 33.6 (2020): 108364. 6. Gajdos, Lukas, et al. "Neutron crystallography reveals mechanisms used by Pseudomonas aeruginosa for host-cell binding." Nature communications 13.1 (2022): 1-9. 7. Yilmaz, Ismail Cem, et al. "Development and preclinical evaluation of virus‐like particle vaccine against COVID‐19 infection." Allergy 77.1 (2022): 258-270. 8. dos Santos Morais, Raphael, et al. "A fast, efficient and easy to implement method to purify bacterial pili from Lacticaseibacillus rhamnosus GG based on multimodal chromatography." Frontiers in Microbiology 11 (2020): 609880. 9. Vagima, Yaron, et al. "Phage therapy potentiates second-line antibiotic treatment against pneumonic plague." Viruses 14.4 (2022): 688. 10. Kawka, Karina, et al. "Comparison of the performance of anion exchange membrane materials for adenovirus purification using laterally-fed membrane chromatography." Biochemical Engineering Journal 182 (2022): 108417. 11. Lefevre, Mathilde, et al. "Sea star-inspired recombinant adhesive proteins self-assemble and adsorb on surfaces in aqueous environments to form cytocompatible coatings." Acta Biomaterialia 112 (2020): 62-74. 12. İpekoğlu, Emre Mert. Development and immunological evaluation of SARS-CoV-2 virus-like particles (VLP) expressing the prefusion stabilized variants of the Spike protein. MS thesis. Middle East Technical University, 2022. 13. Yilmaz, Ismail Cem, et al. "Development and preclinical evaluation of virus‐like particle vaccine against COVID‐19 infection." Allergy 77.1 (2022): 258-270. 14. Wildner, Hendrik, Jean-Charles Paterna, and Karen Haenraets. "Production of AAVs and Injection into the Spinal Cord." Contemporary Approaches to the Study of Pain. Humana, New York, NY, 2022. 375-415. 15. Klaips, Courtney L., et al. "Sis1 potentiates the stress response to protein aggregation and elevated temperature." Nature communications 11.1 (2020): 1-16. 16. Bleuler-Martinez, Silvia, et al. "Structure–function relationship of a novel fucoside-binding fruiting body lectin from Coprinopsis cinerea exhibiting nematotoxic activity." Glycobiology (2022). 17. Gränicher, Gwendal, et al. "A high cell density perfusion process for Modified Vaccinia virus Ankara production: Process integration with inline DNA digestion and cost analysis." Biotechnology and Bioengineering 118.12 (2021): 4720-4734. 18. Labisch, Jennifer J., et al. "Steric exclusion chromatography of lentiviral vectors using hydrophilic cellulose membranes." Journal of Chromatography A (2022): 463148. 19. Martínez-Alarcón, Dania, et al. "SapL1: A New Target Lectin for the Development of Antiadhesive Therapy Against Scedosporium apiospermum." 20. Güvençli, Neşe. PRODUCTION OF VIRUS LIKE PARTICLES EXPRESSING ALPHA (B. 1.1. 7) OR DELTA PLUS (B. 1.617. 2.1) SPIKE VARIANTS AND DEVELOPMENT OF A PSEUDOTYPED VIRUS NEUTRALIZATION ASSAY. MS thesis. Middle East Technical University, 2022. 21. Morais, Raphael Dos Santos, et al. "Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases." Journal of Molecular Biology 434.19 (2022): 167760.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHE16500002-1	41901-1	5×96 Wells	$3872		Add to Cart
BHE16500002-2	41901-2	500mL Washing and dilution buffer (10x)	$70		Add to Cart

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DENARASE® ELISA Kit

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Reviews of DENARASE® ELISA Kit

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