AF488-anti-SAH 1a/1b

Mfr.No.	MAF00301,MAF00301-25,MAF00301-50
Description	Alexa Fluor® 488 (AF488) conjugated anti-S-adenosylhomocysteine monoclonal antibody clone 301-3

Research Area	Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.) One-carbon metabolism Signal Transduction Metabolism Pathways and Processes Cancer Arthritis Neurodegenerative diseases Atherosclerosis Liver diseases Kidney diseases

Dilutions	PBS, pH 7.4, 1% fetal bovine serum or 0.5% BSA, 0.09%NaN3
Purity	>95% purified with Sephadex G-25, free from un-conjugated antibody and Alexa Fluor® 488
Storage	50mM Tris, 150mM NaCl, pH8.0, 0.2% BSA (Sigma), 0.09%NaN3

Applications	FCM

Product Manual	Datasheet

lmmunofluorescence (IF) staining of HepG2 and L02 cells double stained with AF488-anti-SAH 301-3 (Cat# MAF00301) at 20µg/ml and AF647-anti-SAM 118-6 (Cat# MAF00201) at 4µg/ml followed by DAPI staining and photographed under the laser scanning confocal microscope Zeiss LSM 78 (x630). (A1-A4) Normal liver cell line L02 cells cultured in RPMI 1640 with 10% FBS for 40h. Different views are as follows: DAPI (A1); AF488 for SAH (A2); AF647 for SAM (A3); Overlap of all the three fluorescent signals (A4). (B1-B4) Hepatocellular carcinoma cell line HepG2 cells cultured for 40h (100% confluent). Different views are follows: DAPI (B1); AF488 for SAH (B2); AF647 for SAM (B3); Overlap of all the three fluorescent signals (B4). Both antibodies were used at relatively low concentration. When cells are 100% confluent, both SAM and SAH are mainly seen around nuclear membrane.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHE16600059-1	MAF00301-25	25ug	$390		Add to Cart
BHE16600059-2	MAF00301-50	50ug	$690		Add to Cart

AF488-anti-SAH 1a/1b

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